Summary
A continuous cell line was obtained from the culture of embryonic cells of the cupreous chafer, Anomala cuprea Hope. The cells showed substrate-dependent growth and formed loose networks. Population doubling time was about 4.5 d. The mode of chromosome number was about 32 (4n). The cell line was designated FRI-AnCu-35.
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Carlson, J. G. Protoplasmic viscosity changes in different regions of the grass-hopper neuroblast during mitosis. Biol. Bull. 90:109–121; 1946.
Grace, T. D. C. Establishment of four strains of cells from insect tissues grown in vitro. Nature 195:788–789; 1962.
Mitsuhashi, J. Establishment of cell lines from the pupal overies of the swallow tail, Papilio xuthus Linné (Lepidoptera: Papilionidae). Appl. Entomol. Zool. 8:64–72; 1973.
Mitsuhashi, J. A continuous cell line derived from fat bodies of the common armyworm, Leucania separata (Lepidoptera: Noctuidae). Appl. Entomol. Zool. 18:533–539; 1983.
Mitsuhashi, J. A continuously growing cell line from larval hemocytes of Maloacosoma neustria testacea. Jpn. J. Entomol. 64:692–699; 1996.
Mitsuhashi, J. Invertebrate tissue culture methods. Tokyo: Springer-Verlag; 2002.
Mitsuhashi, J.; Inoue, H. Obtainment of a continuous cell line from larval fat bodies of the mulberry tiger moth, Spilosoma imparilis (Lepidoptera, Noctuidae). Appl. Entomol. Zool. 22:533–536; 1988.
Mitsuhashi, J.; Shozawa, A. Continuous cell lines from larval hemocytes of the cabbage armyworm, Mamestra brassicae. Dev. Growth Differ. 27:599–606; 1985.
Takahashi, M.; Mitsuhashi, J.; Ohtaki, T. Establishment of a cell line from embryonic tissues of the fleshfly, Sarcophaga peregrina (Insecta: Diptera). Dev. Growth Differ. 22:11–19; 1980.
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Mitsuhashi, J. A continuous cell line from the cupreous chafer, Anomala cuprea hope (insecta, coleoptera, scarabaeidae). In Vitro Cell.Dev.Biol.-Animal 39, 399–401 (2003). https://doi.org/10.1290/1543-706X(2003)039<0399:ACCLFT>2.0.CO;2
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DOI: https://doi.org/10.1290/1543-706X(2003)039<0399:ACCLFT>2.0.CO;2